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small cell lung cancer cell lines nci h146  (ATCC)


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    ATCC small cell lung cancer cell lines nci h146
    (A) mRNA and western blot of MET expression levels in a series of small cell lung cancer cell lines. (B and C) MET expression by flow cytometry and cytotoxicity in NCI-H1341. N= 3 technical replicates in the cytotoxicity assay; P values from two-way ANOVA. (D-E) MET expression by flow cytometry and cytotoxicity in <t>NCI-H146.</t> N= 3 technical replicates; P values calculated using two-way ANOVA. (F) Histogram of MET expression levels in different cell lines. (G) Cytotoxicity of VHH2-CARs against the same cell lines as in (E). N= 3 technical replicates; P values calculated using two-way ANOVA. * P<0.05; **P<0.01; ***P<0.001; ****P<0.0001 .
    Small Cell Lung Cancer Cell Lines Nci H146, supplied by ATCC, used in various techniques. Bioz Stars score: 95/100, based on 127 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/small+cell+lung+cancer+cell+lines+h146/NCI-H146/bio_rxiv__64898__2026__01__27__702111-36-0-7
    Average 95 stars, based on 127 article reviews
    small cell lung cancer cell lines nci h146 - by Bioz Stars, 2026-09
    95/100 stars

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    1) Product Images from "Nanobody MET CAR T cells show efficacy in solid tumors"

    Article Title: Nanobody MET CAR T cells show efficacy in solid tumors

    Journal: bioRxiv

    doi: 10.64898/2026.01.27.702111

    (A) mRNA and western blot of MET expression levels in a series of small cell lung cancer cell lines. (B and C) MET expression by flow cytometry and cytotoxicity in NCI-H1341. N= 3 technical replicates in the cytotoxicity assay; P values from two-way ANOVA. (D-E) MET expression by flow cytometry and cytotoxicity in NCI-H146. N= 3 technical replicates; P values calculated using two-way ANOVA. (F) Histogram of MET expression levels in different cell lines. (G) Cytotoxicity of VHH2-CARs against the same cell lines as in (E). N= 3 technical replicates; P values calculated using two-way ANOVA. * P<0.05; **P<0.01; ***P<0.001; ****P<0.0001 .
    Figure Legend Snippet: (A) mRNA and western blot of MET expression levels in a series of small cell lung cancer cell lines. (B and C) MET expression by flow cytometry and cytotoxicity in NCI-H1341. N= 3 technical replicates in the cytotoxicity assay; P values from two-way ANOVA. (D-E) MET expression by flow cytometry and cytotoxicity in NCI-H146. N= 3 technical replicates; P values calculated using two-way ANOVA. (F) Histogram of MET expression levels in different cell lines. (G) Cytotoxicity of VHH2-CARs against the same cell lines as in (E). N= 3 technical replicates; P values calculated using two-way ANOVA. * P<0.05; **P<0.01; ***P<0.001; ****P<0.0001 .

    Techniques Used: Western Blot, Expressing, Flow Cytometry, Cytotoxicity Assay

    Related Articles

    Activity Assay:

    Article Title: A Potent and Highly Efficacious Bcl-2/Bcl-xL Inhibitor
    Article Snippet: 25 Cell growth inhibition assay This assay was performed as previously reported.25 Cell growth inhibition assay This assay was performed as previously reported.. 7 Human small cell lung cancer cell lines H146, H1963, H187, and H1417 were purchased from American Type Culture Collection (ATCC) and were maintained in RPMI-1640 medium containing 10% FBS.. Various concentrations of compounds were incubated for 4 days in 96-well flat bottom cell culture plates that were seeded at a density of 1×10 14 cells/well.Various concentrations of compounds were incubated for 4 days in 96-well flat bottom cell culture plates that were seeded at a density of 1×10 14 cells/well.

    Article Title: Structure-based Design of Potent Bcl-2/Bcl-xL Inhibitors with Strong in vivo Antitumor Activity
    Article Snippet: Assays of cell growth and cell death and Western blotting These assays were performed same as previously described.Assays of cell growth and cell death and Western blotting These assays were performed same as previously described.. 17 Human small cell lung cancer cell lines H146 and H1417 were purchased from American Type Culture Collection (ATCC) and were maintained in RPMI-1640 medium containing 10% FBS.. Rabbit antibodies against PARP and caspase-3 are from Cell Signaling Technology.Rabbit antibodies against PARP and caspase-3 are from Cell Signaling Technology.

    Article Title: A Potent and Highly Efficacious Bcl-2/Bcl-xL Inhibitor
    Article Snippet: Millipolarization (mP) values were measured and plotted over total compound concentrations, nonlinear regression fitting to the binding curves generated the IC50 values, and the Ki values were calculated using the equation described previously.25 Cell growth inhibition assay.Millipolarization (mP) values were measured and plotted over total compound concentrations, nonlinear regression fitting to the binding curves generated the IC50 values, and the Ki values were calculated using the equation described previously.25 Cell growth inhibition assay.. This assay was performed as previously reported.7 Human small cell lung cancer cell lines H146, H1963, H187, and H1417 were purchased from American Type Culture Collection (ATCC) and were maintained in RPMI-1640 medium containing 10% FBS.. Various concentrations of compounds were incubated for 4 days in 96-well flat bottom cell culture plates that were seeded at a density of 1x1014 cells/well.Various concentrations of compounds were incubated for 4 days in 96-well flat bottom cell culture plates that were seeded at a density of 1x1014 cells/well.

    Binding Assay:

    Article Title: A Potent and Highly Efficacious Bcl-2/Bcl-xL Inhibitor
    Article Snippet: 25 Cell growth inhibition assay This assay was performed as previously reported.25 Cell growth inhibition assay This assay was performed as previously reported.. 7 Human small cell lung cancer cell lines H146, H1963, H187, and H1417 were purchased from American Type Culture Collection (ATCC) and were maintained in RPMI-1640 medium containing 10% FBS.. Various concentrations of compounds were incubated for 4 days in 96-well flat bottom cell culture plates that were seeded at a density of 1×10 14 cells/well.Various concentrations of compounds were incubated for 4 days in 96-well flat bottom cell culture plates that were seeded at a density of 1×10 14 cells/well.

    Article Title: Structure-based Design of Potent Bcl-2/Bcl-xL Inhibitors with Strong in vivo Antitumor Activity
    Article Snippet: Assays of cell growth and cell death and Western blotting These assays were performed same as previously described.Assays of cell growth and cell death and Western blotting These assays were performed same as previously described.. 17 Human small cell lung cancer cell lines H146 and H1417 were purchased from American Type Culture Collection (ATCC) and were maintained in RPMI-1640 medium containing 10% FBS.. Rabbit antibodies against PARP and caspase-3 are from Cell Signaling Technology.Rabbit antibodies against PARP and caspase-3 are from Cell Signaling Technology.

    Article Title: A Potent and Highly Efficacious Bcl-2/Bcl-xL Inhibitor
    Article Snippet: Millipolarization (mP) values were measured and plotted over total compound concentrations, nonlinear regression fitting to the binding curves generated the IC50 values, and the Ki values were calculated using the equation described previously.25 Cell growth inhibition assay.Millipolarization (mP) values were measured and plotted over total compound concentrations, nonlinear regression fitting to the binding curves generated the IC50 values, and the Ki values were calculated using the equation described previously.25 Cell growth inhibition assay.. This assay was performed as previously reported.7 Human small cell lung cancer cell lines H146, H1963, H187, and H1417 were purchased from American Type Culture Collection (ATCC) and were maintained in RPMI-1640 medium containing 10% FBS.. Various concentrations of compounds were incubated for 4 days in 96-well flat bottom cell culture plates that were seeded at a density of 1x1014 cells/well.Various concentrations of compounds were incubated for 4 days in 96-well flat bottom cell culture plates that were seeded at a density of 1x1014 cells/well.

    Inhibition:

    Article Title: A Potent and Highly Efficacious Bcl-2/Bcl-xL Inhibitor
    Article Snippet: 25 Cell growth inhibition assay This assay was performed as previously reported.25 Cell growth inhibition assay This assay was performed as previously reported.. 7 Human small cell lung cancer cell lines H146, H1963, H187, and H1417 were purchased from American Type Culture Collection (ATCC) and were maintained in RPMI-1640 medium containing 10% FBS.. Various concentrations of compounds were incubated for 4 days in 96-well flat bottom cell culture plates that were seeded at a density of 1×10 14 cells/well.Various concentrations of compounds were incubated for 4 days in 96-well flat bottom cell culture plates that were seeded at a density of 1×10 14 cells/well.

    Article Title: Structure-based Design of Potent Bcl-2/Bcl-xL Inhibitors with Strong in vivo Antitumor Activity
    Article Snippet: Assays of cell growth and cell death and Western blotting These assays were performed same as previously described.Assays of cell growth and cell death and Western blotting These assays were performed same as previously described.. 17 Human small cell lung cancer cell lines H146 and H1417 were purchased from American Type Culture Collection (ATCC) and were maintained in RPMI-1640 medium containing 10% FBS.. Rabbit antibodies against PARP and caspase-3 are from Cell Signaling Technology.Rabbit antibodies against PARP and caspase-3 are from Cell Signaling Technology.

    Article Title: A Potent and Highly Efficacious Bcl-2/Bcl-xL Inhibitor
    Article Snippet: Millipolarization (mP) values were measured and plotted over total compound concentrations, nonlinear regression fitting to the binding curves generated the IC50 values, and the Ki values were calculated using the equation described previously.25 Cell growth inhibition assay.Millipolarization (mP) values were measured and plotted over total compound concentrations, nonlinear regression fitting to the binding curves generated the IC50 values, and the Ki values were calculated using the equation described previously.25 Cell growth inhibition assay.. This assay was performed as previously reported.7 Human small cell lung cancer cell lines H146, H1963, H187, and H1417 were purchased from American Type Culture Collection (ATCC) and were maintained in RPMI-1640 medium containing 10% FBS.. Various concentrations of compounds were incubated for 4 days in 96-well flat bottom cell culture plates that were seeded at a density of 1x1014 cells/well.Various concentrations of compounds were incubated for 4 days in 96-well flat bottom cell culture plates that were seeded at a density of 1x1014 cells/well.

    Western Blot:

    Article Title: A Potent and Highly Efficacious Bcl-2/Bcl-xL Inhibitor
    Article Snippet: 25 Cell growth inhibition assay This assay was performed as previously reported.25 Cell growth inhibition assay This assay was performed as previously reported.. 7 Human small cell lung cancer cell lines H146, H1963, H187, and H1417 were purchased from American Type Culture Collection (ATCC) and were maintained in RPMI-1640 medium containing 10% FBS.. Various concentrations of compounds were incubated for 4 days in 96-well flat bottom cell culture plates that were seeded at a density of 1×10 14 cells/well.Various concentrations of compounds were incubated for 4 days in 96-well flat bottom cell culture plates that were seeded at a density of 1×10 14 cells/well.

    Article Title: Structure-based Design of Potent Bcl-2/Bcl-xL Inhibitors with Strong in vivo Antitumor Activity
    Article Snippet: Assays of cell growth and cell death and Western blotting These assays were performed same as previously described.Assays of cell growth and cell death and Western blotting These assays were performed same as previously described.. 17 Human small cell lung cancer cell lines H146 and H1417 were purchased from American Type Culture Collection (ATCC) and were maintained in RPMI-1640 medium containing 10% FBS.. Rabbit antibodies against PARP and caspase-3 are from Cell Signaling Technology.Rabbit antibodies against PARP and caspase-3 are from Cell Signaling Technology.

    Article Title: A Potent and Highly Efficacious Bcl-2/Bcl-xL Inhibitor
    Article Snippet: Millipolarization (mP) values were measured and plotted over total compound concentrations, nonlinear regression fitting to the binding curves generated the IC50 values, and the Ki values were calculated using the equation described previously.25 Cell growth inhibition assay.Millipolarization (mP) values were measured and plotted over total compound concentrations, nonlinear regression fitting to the binding curves generated the IC50 values, and the Ki values were calculated using the equation described previously.25 Cell growth inhibition assay.. This assay was performed as previously reported.7 Human small cell lung cancer cell lines H146, H1963, H187, and H1417 were purchased from American Type Culture Collection (ATCC) and were maintained in RPMI-1640 medium containing 10% FBS.. Various concentrations of compounds were incubated for 4 days in 96-well flat bottom cell culture plates that were seeded at a density of 1x1014 cells/well.Various concentrations of compounds were incubated for 4 days in 96-well flat bottom cell culture plates that were seeded at a density of 1x1014 cells/well.

    Control:

    Article Title: A Potent and Highly Efficacious Bcl-2/Bcl-xL Inhibitor
    Article Snippet: 25 Cell growth inhibition assay This assay was performed as previously reported.25 Cell growth inhibition assay This assay was performed as previously reported.. 7 Human small cell lung cancer cell lines H146, H1963, H187, and H1417 were purchased from American Type Culture Collection (ATCC) and were maintained in RPMI-1640 medium containing 10% FBS.. Various concentrations of compounds were incubated for 4 days in 96-well flat bottom cell culture plates that were seeded at a density of 1×10 14 cells/well.Various concentrations of compounds were incubated for 4 days in 96-well flat bottom cell culture plates that were seeded at a density of 1×10 14 cells/well.

    Article Title: Structure-based Design of Potent Bcl-2/Bcl-xL Inhibitors with Strong in vivo Antitumor Activity
    Article Snippet: Assays of cell growth and cell death and Western blotting These assays were performed same as previously described.Assays of cell growth and cell death and Western blotting These assays were performed same as previously described.. 17 Human small cell lung cancer cell lines H146 and H1417 were purchased from American Type Culture Collection (ATCC) and were maintained in RPMI-1640 medium containing 10% FBS.. Rabbit antibodies against PARP and caspase-3 are from Cell Signaling Technology.Rabbit antibodies against PARP and caspase-3 are from Cell Signaling Technology.

    Article Title: A Potent and Highly Efficacious Bcl-2/Bcl-xL Inhibitor
    Article Snippet: Millipolarization (mP) values were measured and plotted over total compound concentrations, nonlinear regression fitting to the binding curves generated the IC50 values, and the Ki values were calculated using the equation described previously.25 Cell growth inhibition assay.Millipolarization (mP) values were measured and plotted over total compound concentrations, nonlinear regression fitting to the binding curves generated the IC50 values, and the Ki values were calculated using the equation described previously.25 Cell growth inhibition assay.. This assay was performed as previously reported.7 Human small cell lung cancer cell lines H146, H1963, H187, and H1417 were purchased from American Type Culture Collection (ATCC) and were maintained in RPMI-1640 medium containing 10% FBS.. Various concentrations of compounds were incubated for 4 days in 96-well flat bottom cell culture plates that were seeded at a density of 1x1014 cells/well.Various concentrations of compounds were incubated for 4 days in 96-well flat bottom cell culture plates that were seeded at a density of 1x1014 cells/well.

    Injection:

    Article Title: A Potent and Highly Efficacious Bcl-2/Bcl-xL Inhibitor
    Article Snippet: 25 Cell growth inhibition assay This assay was performed as previously reported.25 Cell growth inhibition assay This assay was performed as previously reported.. 7 Human small cell lung cancer cell lines H146, H1963, H187, and H1417 were purchased from American Type Culture Collection (ATCC) and were maintained in RPMI-1640 medium containing 10% FBS.. Various concentrations of compounds were incubated for 4 days in 96-well flat bottom cell culture plates that were seeded at a density of 1×10 14 cells/well.Various concentrations of compounds were incubated for 4 days in 96-well flat bottom cell culture plates that were seeded at a density of 1×10 14 cells/well.

    Article Title: Structure-based Design of Potent Bcl-2/Bcl-xL Inhibitors with Strong in vivo Antitumor Activity
    Article Snippet: Assays of cell growth and cell death and Western blotting These assays were performed same as previously described.Assays of cell growth and cell death and Western blotting These assays were performed same as previously described.. 17 Human small cell lung cancer cell lines H146 and H1417 were purchased from American Type Culture Collection (ATCC) and were maintained in RPMI-1640 medium containing 10% FBS.. Rabbit antibodies against PARP and caspase-3 are from Cell Signaling Technology.Rabbit antibodies against PARP and caspase-3 are from Cell Signaling Technology.

    Article Title: A Potent and Highly Efficacious Bcl-2/Bcl-xL Inhibitor
    Article Snippet: Millipolarization (mP) values were measured and plotted over total compound concentrations, nonlinear regression fitting to the binding curves generated the IC50 values, and the Ki values were calculated using the equation described previously.25 Cell growth inhibition assay.Millipolarization (mP) values were measured and plotted over total compound concentrations, nonlinear regression fitting to the binding curves generated the IC50 values, and the Ki values were calculated using the equation described previously.25 Cell growth inhibition assay.. This assay was performed as previously reported.7 Human small cell lung cancer cell lines H146, H1963, H187, and H1417 were purchased from American Type Culture Collection (ATCC) and were maintained in RPMI-1640 medium containing 10% FBS.. Various concentrations of compounds were incubated for 4 days in 96-well flat bottom cell culture plates that were seeded at a density of 1x1014 cells/well.Various concentrations of compounds were incubated for 4 days in 96-well flat bottom cell culture plates that were seeded at a density of 1x1014 cells/well.



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    small cell lung cancer cell lines nci h146  (ATCC)
    95
    ATCC small cell lung cancer cell lines nci h146
    (A) mRNA and western blot of MET expression levels in a series of small cell lung cancer cell lines. (B and C) MET expression by flow cytometry and cytotoxicity in NCI-H1341. N= 3 technical replicates in the cytotoxicity assay; P values from two-way ANOVA. (D-E) MET expression by flow cytometry and cytotoxicity in <t>NCI-H146.</t> N= 3 technical replicates; P values calculated using two-way ANOVA. (F) Histogram of MET expression levels in different cell lines. (G) Cytotoxicity of VHH2-CARs against the same cell lines as in (E). N= 3 technical replicates; P values calculated using two-way ANOVA. * P<0.05; **P<0.01; ***P<0.001; ****P<0.0001 .
    Small Cell Lung Cancer Cell Lines Nci H146, supplied by ATCC, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/small+cell+lung+cancer+cell+lines+h146/NCI-H146/bio_rxiv__64898__2026__01__27__702111-36-0-7
    Average 95 stars, based on 1 article reviews
    small cell lung cancer cell lines nci h146 - by Bioz Stars, 2026-09
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    small cell lung cancer cell lines h146  (ATCC)
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    ATCC small cell lung cancer cell lines h146
    Antitumor activity of compounds 31 and 32 in the <t>H146</t> small-cell lung cancer xenograft model in SCID mice. Tumors were grown to an average size of 126 mm3 and compound 31 or 32 was administered at 15 mg/kg intravenously, daily, 5 days a week for 2 weeks. (a). Tumor growth. (b). Animal body weight.
    Small Cell Lung Cancer Cell Lines H146, supplied by ATCC, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/small+cell+lung+cancer+cell+lines+h146/NCI-H146%3B+Small+Cell+Lung+Cancer%3B+Human/pmc03806060-492-2-16
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    small cell lung cancer cell lines h146 - by Bioz Stars, 2026-09
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    human small cell lung cancer cell lines h146  (ATCC)
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    ATCC human small cell lung cancer cell lines h146
    Binding affinities of our designed compounds to Bcl-2 and Bcl-X L proteins in our FP-based assays and inhibition of cell growth in two cancer cell lines.
    Human Small Cell Lung Cancer Cell Lines H146, supplied by ATCC, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/small+cell+lung+cancer+cell+lines+h146/NCI-H146%3B+Small+Cell+Lung+Cancer%3B+Human/pmc03397176-557-0-14
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    human smallcell lung cancer cell lines h146  (ATCC)
    93
    ATCC human smallcell lung cancer cell lines h146
    Figure 7. (A) Representative cell growth inhibition of 12, 14, 15, and 1 in three small-cell lung cancer cell lines. (B) Cell death induction by 12, 14, 15, and 1 in <t>H146</t> cell line. Cells were treated for 24 h, and cell death was analyzed by trypan blue assay. (C) Induction of cleavage of PARP and caspase-3 in H146 cell line by 12, 14, 15, and 1. Cells were treated for 24 h, and caspase-3 (Cas 3) and PARP were probed by Western blotting: Cl PARP, cleaved PARP; Cl Cas 3, cleaved caspase-3. GAPDH was used as the loading control.
    Human Smallcell Lung Cancer Cell Lines H146, supplied by ATCC, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/small+cell+lung+cancer+cell+lines+h146/NCI-H146%3B+Small+Cell+Lung+Cancer%3B+Human/10__1021_slash_jm300608w-322-7-19
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    (A) mRNA and western blot of MET expression levels in a series of small cell lung cancer cell lines. (B and C) MET expression by flow cytometry and cytotoxicity in NCI-H1341. N= 3 technical replicates in the cytotoxicity assay; P values from two-way ANOVA. (D-E) MET expression by flow cytometry and cytotoxicity in NCI-H146. N= 3 technical replicates; P values calculated using two-way ANOVA. (F) Histogram of MET expression levels in different cell lines. (G) Cytotoxicity of VHH2-CARs against the same cell lines as in (E). N= 3 technical replicates; P values calculated using two-way ANOVA. * P<0.05; **P<0.01; ***P<0.001; ****P<0.0001 .

    Journal: bioRxiv

    Article Title: Nanobody MET CAR T cells show efficacy in solid tumors

    doi: 10.64898/2026.01.27.702111

    Figure Lengend Snippet: (A) mRNA and western blot of MET expression levels in a series of small cell lung cancer cell lines. (B and C) MET expression by flow cytometry and cytotoxicity in NCI-H1341. N= 3 technical replicates in the cytotoxicity assay; P values from two-way ANOVA. (D-E) MET expression by flow cytometry and cytotoxicity in NCI-H146. N= 3 technical replicates; P values calculated using two-way ANOVA. (F) Histogram of MET expression levels in different cell lines. (G) Cytotoxicity of VHH2-CARs against the same cell lines as in (E). N= 3 technical replicates; P values calculated using two-way ANOVA. * P<0.05; **P<0.01; ***P<0.001; ****P<0.0001 .

    Article Snippet: Small cell lung cancer cell lines NCI-H146 (ATCC HB173) and NCI-H1341(ATCC CRL-5864) were cultured in DMEM-F12 supplemented with 10% FBS, 1X Insulin-Transferrin-Selenium and 1% penicillin/streptomycin.

    Techniques: Western Blot, Expressing, Flow Cytometry, Cytotoxicity Assay

    Antitumor activity of compounds 31 and 32 in the H146 small-cell lung cancer xenograft model in SCID mice. Tumors were grown to an average size of 126 mm3 and compound 31 or 32 was administered at 15 mg/kg intravenously, daily, 5 days a week for 2 weeks. (a). Tumor growth. (b). Animal body weight.

    Journal: Journal of medicinal chemistry

    Article Title: A Potent and Highly Efficacious Bcl-2/Bcl-xL Inhibitor

    doi: 10.1021/jm4001105

    Figure Lengend Snippet: Antitumor activity of compounds 31 and 32 in the H146 small-cell lung cancer xenograft model in SCID mice. Tumors were grown to an average size of 126 mm3 and compound 31 or 32 was administered at 15 mg/kg intravenously, daily, 5 days a week for 2 weeks. (a). Tumor growth. (b). Animal body weight.

    Article Snippet: 7 Human small cell lung cancer cell lines H146, H1963, H187, and H1417 were purchased from American Type Culture Collection (ATCC) and were maintained in RPMI-1640 medium containing 10% FBS.

    Techniques: Activity Assay

    Binding affinities of our designed compounds to Bcl-2 and Bcl-X L proteins in our FP-based assays and inhibition of cell growth in two cancer cell lines.

    Journal: Journal of Medicinal Chemistry

    Article Title: Design of Bcl-2 and Bcl-xL Inhibitors with Subnanomolar Binding Affinities Based Upon a New Scaffold

    doi: 10.1021/jm300178u

    Figure Lengend Snippet: Binding affinities of our designed compounds to Bcl-2 and Bcl-X L proteins in our FP-based assays and inhibition of cell growth in two cancer cell lines.

    Article Snippet: Human small cell lung cancer cell lines H146 and H1417 were purchased from the American Type Culture Collection (ATCC) and were maintained in RPMI-1640 medium containing 10% FBS.

    Techniques: Binding Assay, Inhibition

    Binding affinities of our designed compounds to Bcl-2 and Bcl-X L proteins in FP-based assays and inhibition of cell growth in two small-cell lung cancer cell lines.

    Journal: Journal of Medicinal Chemistry

    Article Title: Design of Bcl-2 and Bcl-xL Inhibitors with Subnanomolar Binding Affinities Based Upon a New Scaffold

    doi: 10.1021/jm300178u

    Figure Lengend Snippet: Binding affinities of our designed compounds to Bcl-2 and Bcl-X L proteins in FP-based assays and inhibition of cell growth in two small-cell lung cancer cell lines.

    Article Snippet: Human small cell lung cancer cell lines H146 and H1417 were purchased from the American Type Culture Collection (ATCC) and were maintained in RPMI-1640 medium containing 10% FBS.

    Techniques: Binding Assay, Inhibition

    (A). Cell death induction by 20 and 21 in the H146 cancer cell line. Cells were treated for 24 h and cell death was analyzed by trypan blue assay. (B). Induction of cleavage of PARP and caspase-3 by 20 and 21 in the H146 cell line. Cells were treated for 24 h and caspase-3 and PARP were probed by western blotting.

    Journal: Journal of Medicinal Chemistry

    Article Title: Design of Bcl-2 and Bcl-xL Inhibitors with Subnanomolar Binding Affinities Based Upon a New Scaffold

    doi: 10.1021/jm300178u

    Figure Lengend Snippet: (A). Cell death induction by 20 and 21 in the H146 cancer cell line. Cells were treated for 24 h and cell death was analyzed by trypan blue assay. (B). Induction of cleavage of PARP and caspase-3 by 20 and 21 in the H146 cell line. Cells were treated for 24 h and caspase-3 and PARP were probed by western blotting.

    Article Snippet: Human small cell lung cancer cell lines H146 and H1417 were purchased from the American Type Culture Collection (ATCC) and were maintained in RPMI-1640 medium containing 10% FBS.

    Techniques: Western Blot

    Figure 7. (A) Representative cell growth inhibition of 12, 14, 15, and 1 in three small-cell lung cancer cell lines. (B) Cell death induction by 12, 14, 15, and 1 in H146 cell line. Cells were treated for 24 h, and cell death was analyzed by trypan blue assay. (C) Induction of cleavage of PARP and caspase-3 in H146 cell line by 12, 14, 15, and 1. Cells were treated for 24 h, and caspase-3 (Cas 3) and PARP were probed by Western blotting: Cl PARP, cleaved PARP; Cl Cas 3, cleaved caspase-3. GAPDH was used as the loading control.

    Journal: Journal of Medicinal Chemistry

    Article Title: Structure-Based Design of Potent Bcl-2/Bcl-xL Inhibitors with Strong in Vivo Antitumor Activity

    doi: 10.1021/jm300608w

    Figure Lengend Snippet: Figure 7. (A) Representative cell growth inhibition of 12, 14, 15, and 1 in three small-cell lung cancer cell lines. (B) Cell death induction by 12, 14, 15, and 1 in H146 cell line. Cells were treated for 24 h, and cell death was analyzed by trypan blue assay. (C) Induction of cleavage of PARP and caspase-3 in H146 cell line by 12, 14, 15, and 1. Cells were treated for 24 h, and caspase-3 (Cas 3) and PARP were probed by Western blotting: Cl PARP, cleaved PARP; Cl Cas 3, cleaved caspase-3. GAPDH was used as the loading control.

    Article Snippet: These assays were performed as previously described.17 Human smallcell lung cancer cell lines H146 and H1417 were purchased from American Type Culture Collection (ATCC) and were maintained in dx.doi.org/10.1021/jm300608w | J. Med.

    Techniques: Inhibition, Western Blot, Control

    Figure 8. Analysis of cytochrome c (Cyto c) release from mitochondria into cytosol and cleavage of PARP (Cl PARP) induced by 14 and 15 in H146 cells in 2 h. GAPDH was used as the loading control.

    Journal: Journal of Medicinal Chemistry

    Article Title: Structure-Based Design of Potent Bcl-2/Bcl-xL Inhibitors with Strong in Vivo Antitumor Activity

    doi: 10.1021/jm300608w

    Figure Lengend Snippet: Figure 8. Analysis of cytochrome c (Cyto c) release from mitochondria into cytosol and cleavage of PARP (Cl PARP) induced by 14 and 15 in H146 cells in 2 h. GAPDH was used as the loading control.

    Article Snippet: These assays were performed as previously described.17 Human smallcell lung cancer cell lines H146 and H1417 were purchased from American Type Culture Collection (ATCC) and were maintained in dx.doi.org/10.1021/jm300608w | J. Med.

    Techniques: Control

    Figure 9. Analysis of induction of cleavage of PARP and caspase-3 in the H146 xenograft tumor tissues by 14 (25 mg/kg, iv). Mice bearing H146 xenograft tumors were dosed with either 14 or vehicle. Animals were sacrificed at 3, 6, and 24 h time points, and tumor tissues were analyzed by Western blot for cleavage of PARP (Cl PARP) and caspase-3 (Cl caspase-3). GAPDH was used as the loading control.

    Journal: Journal of Medicinal Chemistry

    Article Title: Structure-Based Design of Potent Bcl-2/Bcl-xL Inhibitors with Strong in Vivo Antitumor Activity

    doi: 10.1021/jm300608w

    Figure Lengend Snippet: Figure 9. Analysis of induction of cleavage of PARP and caspase-3 in the H146 xenograft tumor tissues by 14 (25 mg/kg, iv). Mice bearing H146 xenograft tumors were dosed with either 14 or vehicle. Animals were sacrificed at 3, 6, and 24 h time points, and tumor tissues were analyzed by Western blot for cleavage of PARP (Cl PARP) and caspase-3 (Cl caspase-3). GAPDH was used as the loading control.

    Article Snippet: These assays were performed as previously described.17 Human smallcell lung cancer cell lines H146 and H1417 were purchased from American Type Culture Collection (ATCC) and were maintained in dx.doi.org/10.1021/jm300608w | J. Med.

    Techniques: Western Blot, Control

    Figure 10. Antitumor activity of compound 14 in H146 xenograft model. H146 tumor cells were injected subcutaneously into SCID mice, and treatments started when tumors reached a mean volume of 70 mm3. Each group consisted of eight mice/tumors.

    Journal: Journal of Medicinal Chemistry

    Article Title: Structure-Based Design of Potent Bcl-2/Bcl-xL Inhibitors with Strong in Vivo Antitumor Activity

    doi: 10.1021/jm300608w

    Figure Lengend Snippet: Figure 10. Antitumor activity of compound 14 in H146 xenograft model. H146 tumor cells were injected subcutaneously into SCID mice, and treatments started when tumors reached a mean volume of 70 mm3. Each group consisted of eight mice/tumors.

    Article Snippet: These assays were performed as previously described.17 Human smallcell lung cancer cell lines H146 and H1417 were purchased from American Type Culture Collection (ATCC) and were maintained in dx.doi.org/10.1021/jm300608w | J. Med.

    Techniques: Activity Assay, Injection